GC-MS data files of the analysis of Coelopa frigida cuticular hydrocarbons as cdf files. 
Please read "Metadata for GC-MS CHC analysis.xls" for information on populations, sex, genotype, fly weight and and note the specific comments to each data file.

Description of GC-MS aquisition method:
Frozen flies were allowed to defrost and dry for 10 minutes. 
Each fly was then placed in a 1.5 ml high recovery vial containing 300 µl of n-hexane, vortexed at a low 
speed for 5 seconds, and extracted for 5 minutes. Afterwards flies were removed from the vial and allowed 
to air dry before they were weighed on a Sartorius Quintix 124-1S microbalance to the nearest 0.0001 grams. 
Extracts were evaporated until dryness under a stream of nitrogen and stored at -20° until GC-MS analysis. 
Before analysis, extracts were redissolved in 20 µl of n-hexane containing 1 µg/ml n-nonane (Sigma-Aldrich) 
as an internal standard and vortexed at maximum speed for 10 seconds. Fly extracts were analyzed on a 
GC (Agilent GC 6890) coupled to a MS (Agilent 5973 MSD) using a HP-5MS capillary column (Agilent, 30m x 0.25mm ID, 
0.25µm film thickness). Two microliters were injected in a pulsed splitless mode. 
Inlet and MSD transfer line temperature was set isothermal to 280°C.  
The oven temperature was programmed to 190°C for 3 min, increased to 280˚C at a rate of 8˚C/min, 
then increased to 325˚C at a rate 4˚C/min and finally held at this temperature for 14.5 min. 
Helium was used as the carrier gas at a constant flow of 0.5 ml/min. 
The mass spectrometer was operated in scan mode scanning ions from 40–700 amu (2.24 scans s−1) with an electron 
ionization at 70 eV, a source temperature at 230°C and quadrupole temperature at 150°C and an initial 3 min solvent delay.  